Plant Molecular Biology Manual

<em>Plant Molecular Biology Manual</em> (Second Edition) is an entirely new manual containing both fundamental and recently described techniques in the area of plant molecular biology. Designed for use in the research laboratory, the <em>Plant Molecular Biology Manual</em> presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and <em>in vitro</em> transcription systems, <em>in situ</em> hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized <em>Agrobacterium</em> strains and T-DNA vectors, nuclear run-on and <em>in vitro</em> transcription systems, non-radioactive detection systems, characterization of transcription factors, nuclear protein targeting, and T-DNA and transposon mutagenesis, not previously described in the first edition.