Screening of RGA Marker(s) in Mungbean Against Yellow Mosaic Disease

Using the disease grading system, 100 genotypes of mugbeans were tested in the field for yellow mosaic disease resistance during Rabi 2021-2022. The yellow mosaic disease was resistant to 49 of the 100 mugbean genotypes, Moderately resistant to 22, Moderately susceptible to 11, susceptible 3, and Highly susceptible to 15 of the genotypes. According to BLAST tool results, the sequenced virus has a 98.94% similarity to the entire coding sequence of the MYMIV-Mb02 coat protein (AV1) gene from the mungbean yellow mosaic India virus strain, accession number GQ387502.1. Consequently, the variant was given the accession number ON622515.1 and named mungbean yellow mosaic India virus isolates NAU-RJ coat protein gene segment. Six resistant (40 C, NMS-21-01, NMS-21-06, NMS-21-22, NMS-21-49, NMS-21-95) and six highly susceptible (GM 4, NMS-21-23, NMS-21-24, NMS-21-40, NMS-21-68, NMS-21-69), were studied in the current study. Nine pairs of RGA primers were used to screen six resistant and six susceptible genotypes of mungbean. This resulted in effective amplification of five pairs of RGA primers in all resistant genotypes, but not in all highly susceptible genotypes.