Isolation

Scientific Study from the year 2016 in the subject Biology - Micro- and Molecular Biology, grade: 1.5, Mar Augusthinose College, language: English, abstract: In this study an attempt was made to evaluate the colour degradation capabilities by collecting the contaminated soil sample from Kalady area and serial dilution was done upto 10-6. From the dilution 10-5 was taken and spread plated on Nutrient agar. From the above plate, isolated colonies was obtained which was found to be Bacillus sp and Pseudomonas sp respectively by morphological, microscopical and biochemical method. The isolated colonies was taken for degradation studies with 1% dye and 1% inoculum in Nutrient broth and OD values and colour change was noted. It was found to be Bacillus sp has more degrading capacity in yellow colour than Pseudmonas sp. The optimization studies was done with Bacillus sp having different concentration of colour (2, 4, 6) with varying pH (4,6,8) and temperature (37 C, 40 C and room temperature). The result was found to be having the concentration of colour with 4% having pH 4 and temperature 37 C.