Isolation and identification of the Newcastle disease virus

Seminar paper from the year 2016 in the subject Veterinary medicine, Addis Ababa University, language: English, abstract: Newcastle disease (NCD) is a contagious bird disease affecting domestic and wild avian species characterised by respiratory signs (gasping, coughing), nervous signs depression, in appetence, muscular tremors, and drooping wings, twisting of head and neck, greenish watery diarrhoea, misshapen, rough-or thin shelled eggs and reduced egg production. This study was designed for isolation and identification of Newcastle disease virus from sick and suspected chicken in poultry farm, Bishoftu, Ethiopia, during October 16, 2015 - May 30, 2016 which brought to research and diagnostic laboratory in National Veterinary Institute (NVI), Ethiopia by owner. Total suspected chicken were 200 and out of these 30 chickens were brought to NVI and samples were collected. The sample included serum, cloacal and tracheal swab. Serum sample was collected for confirmatory diagnosis by serological HI test. HI result has showed that more than 80% of the total samples collected were positive for Newcastle disease virus (NCDV). Isolation of the virus from cloacal and tracheal swabs was performed by inoculating each suspected sample in to 10 day old embryonating eggs. Out of 30 NCDV suspected samples 24 (6layers and 18broilers) were positive for NCD virus isolation. Out of the 24 HA positive NCD virus suspected samples 24(6 layers and 18broilers were HI positive. Keywords: Diseases, Haemagglutination, Inhibition. Isolation, Newcastle, Virus