Determination of Venlafaxine hydrochloride by UV spectrophotometry

UV Spectrophotometric determination of Venlafaxine Hydrochloride was done by using Distilled water as a solvent to dissolve the drug(pure) with a known concentration (10 g/ml). λmax was determined which showed maximum absorbance at 224 nm. It obeys Beers Lamberts law by showing linearity at concentration range 2 - 14 g/ml. By under going the analysis of formulation we concluded that it has maximum active ingredient in solid dosage form tablets of Venlafaxine Hydrochloride. The percentage purity of Venlafaxine Hydrochloride tablets was determined by performing assay and it was found to be 96.09 % w/w. The percentage recovery is observed in the range of 101.5 % w/w which indicates no interference of the excipients present in the tablet formulation. Precision was determined by under going repeatability studies where absorbance was almost same. Intraday precision was performed by taking the absorbance of the same concentration at several times in a day and its % RSD was minimum as per ICH guidelines. Same absorbance was seen for same concentration i.e., 10 g/ml when observed on different days by which we determined the interday. Stability was kept unchanged up to 5hrs.